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This article waslast modified on 19 April 2021.

What are genetic tests for targeted cancer therapy?

Genetic tests for targeted cancer therapy detect mutations (changes) in the DNA of cancer cells. Knowing whether the cancer has a particular mutation can help guide the type of treatment that a person receives. The presence or absence of certain mutations can predict who may benefit from certain drugs and who is not likely to respond.

Cancer is the uncontrolled growth of abnormal cells. Multiple factors may contribute to this uncontrolled growth. One such factor is the malfunctioning of proteins involved in controlling cell growth and maturation. The proteins usually malfunction as a result of a mutation in the DNA of the gene that codes for that protein. Some mutations may result in a defective protein that cannot stop cell growth while other mutations may produce a protein with altered function that stimulates cell growth. The net result is unchecked growth and division of these abnormal cells (cancer).

Medical researchers have long studied these changes in genes in order to better understand cancer and to develop drugs to fight it. Their goal has been to create drugs that disrupt a specific step in cancer growth, while doing minimal damage to normal cells. These are called targeted drugs or targeted therapy. What researchers have noted is that specific types of cancer are frequently associated with specific genetic mutations. Not every cancer will have them, but a significant percentage will, and cancers with these mutations usually have a more predictable response to certain drug treatments compared to cancers without these mutations.

These findings have led to two important developments:

  • Cancer drugs that inhibit or target very specific proteins associated with certain cancers (two examples are tyrosine kinase inhibitors and epidermal growth factor receptor (EGFR) antibodies.)
  • Genetic tests to detect the presence of mutations in cancer tissue that tell a healthcare practitioner whether the person being tested is likely to benefit from a specific therapy

Medical researchers continue to explore the genetics of cancer and to look for opportunities to develop new therapies. Additionally, some cancers eventually stop responding to certain therapies and develop resistance to that therapy. Genetic research may offer insights into how resistance to therapy occurs.

Why is this testing important?

Standard treatment for cancer usually involves surgery, chemotherapy, radiation therapy, or some combination of these. Treatment with chemotherapy drugs and radiation aims to slow the growth of cancer, keep it from spreading, and kill any cancerous cells that have spread to other parts of the body (metastasized).

Chemotherapy works by attacking cells that are actively growing and dividing. Radiation therapy kills cancer cells by damaging their genes and preventing them from growing and dividing. Both types of therapy can affect all cells that are growing and dividing, including normal cells. This often leads to harmful side effects, and these treatments require careful adjustment to maximize the killing of cancer cells while minimizing the damage to healthy tissue.

Targeted therapy is a newer type of cancer treatment that offers healthcare practitioners and their patients the opportunity to use a drug that has a greater effect on cancerous tissue, reducing many of the side effects associated with standard therapy. It is based on the fact that the genetic makeup of the cancer cells is different than the normal cells around them. Targeted therapy aims to disrupt specific steps or processes that are somewhat unique to the growth of cancer cells. Testing the cancer cells taken from patients in a biopsy prior to initiating drug therapy may be necessary to determine the cancer's likely response to certain drugs.

Targeted cancer drugs are expensive, and they generally only work in patients whose cancer has the genetic makeup that they have been developed to work against. Genetic testing prior to beginning therapy is necessary to match the treatment up with the patients and cancers likely to benefit from them.

Examples of targeted cancer drugs for which tests are available include:

  • Drugs that bind to receptors on the cell surface and block growth signalling to the cell
  • Drugs that are small molecules that cross the cell membrane and block the signals for growth at the receptor's active site

On average it takes 7 working days for the blood test results to come back from the hospital, depending on the exact tests requested. Some specialist test results may take longer, if samples have to be sent to a reference (specialist) laboratory. The X-ray & scan results may take longer. If you are registered to use the online services of your local practice, you may be able to access your results online. Your GP practice will be able to provide specific details.

If the doctor wants to see you about the result(s), you will be offered an appointment. If you are concerned about your test results, you will need to arrange an appointment with your doctor so that all relevant information including age, ethnicity, health history, signs and symptoms, laboratory and other procedures (radiology, endoscopy, etc.), can be considered.

Lab Tests Online-UK is an educational website designed to provide patients and carers with information on laboratory tests used in medical care. We are not a laboratory and are unable to comment on an individual's health and treatment.

Reference ranges are dependent on many factors, including patient age, sex, sample population, and test method, and numeric test results can have different meanings in different laboratories.

For these reasons, you will not find reference ranges for the majority of tests described on this web site. The lab report containing your test results should include the relevant reference range for your test(s). Please consult your doctor or the laboratory that performed the test(s) to obtain the reference range if you do not have the lab report.

For more information on reference ranges, please read Reference Ranges and What They Mean.


How is genetic testing for targeted cancer therapy used?

These genetic tests are used to help guide treatment for certain cancers. They help to inform a healthcare practitioner as to whether certain targeted cancer drugs may or may not work.

Genes are the basic units of genetic material, the segments of DNA that usually code for the production of specific proteins. Alterations in DNA are called genetic variants (also polymorphisms or mutations) and occur throughout the population. Variants or mutations are largely inherited and affect all cells, but they can occur later in life, because of exposures to radiation, toxins, or for unknown reasons, and these mutations may result in cancer.

In a variety of cancers, there may be a mutation that leads to an increased amount of a particular protein present in the tumour tissue or to production of a protein that has altered activity. Tumours that have these mutations may tend to grow more aggressively, be more likely to spread (metastasize), and/or may be more resistant to standard chemotherapy. Sometimes, however, the changes in the protein also make the tumours candidates for therapy that targets the changed protein ("targeted therapy"). Genetic tests for cancer therapy detect the mutations that code for these proteins, thus identifying tumours that may be susceptible to targeted therapy.

Conversely, genetic tests may also identify tumours that will not respond to targeted therapy. Certain mutations, when present, make the cancer cells resistant to the drug and targeted therapy will not be used for treatment.

When are the tests requested?

Testing may be ordered as part of an initial workup of particular cancers or performed on those with certain cancers that are not responding to chemotherapy. It requires a sample of the tumour tissue, and if a sample is available from a previous biopsy used for diagnosis, it can be done on that sample.

The tests are usually performed only once. However, testing may be done more than once if a patient's tumour progresses while on therapy to see if the tumour has acquired mutations that are resistant to the therapy.


Each genetic test for a specific targeted cancer therapy identifies mutations in a single gene, and test results are specific to the gene and the targeted therapies being evaluated.

The table below lists examples of some cancers for which genetic testing may be used to help make decisions about targeted drug therapy.

Type of Cancer

Gene Tested*

Interpretation of Test Result

Breast cancer


When present, likely response to trastuzumab

Chronic myelogenous leukaemia (CML)


Nonresponsive to imatinib when mutation(s) present



When present, can be measured periodically to monitor response to targeted drug

Bowel cancer


When mutation present, likely resistance to tyrosine kinase inhibitor



Poor prognosis when mutation present

Gastrointestinal stromal tumour (GIST)—rare tumours of the digestive tract


Depending on mutation present, better response to imatinib therapy, increased dose of imatinib likely necessary and better response to sunitinib, or possible resistance to imatinib



When mutation present, less likely to respond to imatinib



Better response to vemurafenib when mutation present with metastatic melanoma

Myeloproliferative neoplasms (MPNs)


When mutation present, may be measured periodically to monitor responsiveness to treatment (e.g., Ruxolitinib)

Non-small cell lung cancer (NSCLC)


Best response to tyrosine kinase inhibitors such as gefitinib and erlotinib in those with certain mutations



If ALK is present, may respond to ALK kinase inhibitors, such as crizotinib



If ROS1 is present, ALK kinase inhibitors, such as crizotinib



Poorer prognosis when certain mutations present, resistance to tyrosine kinase inhibitors, and poor response to platinum/vinorelbine therapy



Likely response to immunotherapy

Cancers of unknown origin—cancers detected in unusual body sites and thought to have spread (metastasized) from another location

Several genes evaluated together (genomic array or profile)

Helps determine the organ or body part in which the cancer originated in order to help guide treatment

* Gene names are typically abbreviated for ease of use because full names are often several words long.

Usually, the cancer drugs and genetic tests listed in the table above have been developed concurrently and the tests are referred to as companion diagnostics. These are laboratory tests that are developed specifically to provide information that is essential for the safe and effective use of a corresponding therapeutic product. In many cases, results from these tests are needed for healthcare practitioners to be able to make decisions regarding treatment of their patients.

Cancers associated with a strong family history and those that occur at a young age may have different characteristics than those that develop sporadically in adults. For instance, paediatric cases of GIST are very different to adult cases and do not typically have KIT or PDGFRA mutations.

Only common mutations are tested. A negative test result does not rule out the possibility that a person has a less common mutation. To rule out the possibility that the mutation was not present in the sample tested, additional samples may be needed.

Some tests for specific gene mutations in certain types of cancer are available on a limited basis and/or not used routinely for medical purposes. These genetic tests may, however, be used in research settings and their utility in medical care may evolve as research progresses. Some examples include:

  • Colon cancer: PIK3CA and NRAS
  • Melanoma: KIT and NRAS
  • Myeloproliferative neoplasms: PDGFRA
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