Also Known As
E. coli
E. coli O157
E. coli non-O157
Formal Name
E. coli O157 culture; Shiga-like toxin by EIA; Stx gene detection by PCR
This article was last reviewed on
This article waslast modified on 14 February 2023.
At a Glance
Why Get Tested?

To determine if your gastrointestinal symptoms are due to an infection caused by Escherichia coli bacteria that produce the Shiga toxin

When To Get Tested?

1. When you have acute diarrhoea that is persistent, severe and/or bloody.
2. Your doctor may decide to test if you have a condition called Haemolytic Uraemic syndrome (HUS).
3. Your doctor or public health team may want to test in some cases if you came in to close contact with a patient with an infection caused by Shiga toxin-producing Escherichia coli or HUS.

Sample Required?

A fresh liquid or unformed stool sample that does not contain urine or water, collected into a clean dry container; a rectal swab may be collected from infants. Stool and rectal swab may be placed in transport media for delivery to laboratory.

Test Preparation Needed?


On average it takes 7 working days for the blood test results to come back from the hospital, depending on the exact tests requested. Some specialist test results may take longer, if samples have to be sent to a reference (specialist) laboratory. The X-ray & scan results may take longer. If you are registered to use the online services of your local practice, you may be able to access your results online. Your GP practice will be able to provide specific details.

If the doctor wants to see you about the result(s), you will be offered an appointment. If you are concerned about your test results, you will need to arrange an appointment with your doctor so that all relevant information including age, ethnicity, health history, signs and symptoms, laboratory and other procedures (radiology, endoscopy, etc.), can be considered.

Lab Tests Online-UK is an educational website designed to provide patients and carers with information on laboratory tests used in medical care. We are not a laboratory and are unable to comment on an individual's health and treatment.

Reference ranges are dependent on many factors, including patient age, sex, sample population, and test method, and numeric test results can have different meanings in different laboratories.

For these reasons, you will not find reference ranges for the majority of tests described on this web site. The lab report containing your test results should include the relevant reference range for your test(s). Please consult your doctor or the laboratory that performed the test(s) to obtain the reference range if you do not have the lab report.

For more information on reference ranges, please read Reference Ranges and What They Mean.

What is being tested?

These tests detect the presence of disease-causing (pathogenic) Escherichia coli that produce Shiga toxins. E. coli bacteria commonly occur in nature and they are a necessary component of the digestive process in humans and most other mammals. Most strains of E. coli are harmless, but pathogenic E. coli can be responsible for inflammation of the bowel (gastroenteritis). There are multiple subtypes of E. coli that cause diarrhoeal illness, and they are classified by the mechanisms by which they cause disease. For example, some invade the lining of the intestines, causing inflammation, while others produce toxins.

E. coli that produce poisons called Shiga toxins are generally the only type of E. coli that are tested for in clinical settings from stool specimens. The Shiga toxins associated with these infections are so called because they are related to the toxins produced by another type of disease-causing bacteria, Shigella.

Outbreaks have been linked to the consumption of contaminated food, including undercooked minced beef, unpasteurised juice, raw milk, and raw produce such as leafy greens and alfalfa sprouts. Shiga toxin-producing E. Coli (STEC) may also be transmitted through contaminated water, contact with farm animals or their environment, and from person to person.

In addition to symptoms of nausea, severe abdominal cramps, watery diarrhoea, fatigue, or possible vomiting and low-grade fever, STEC infections are often associated with bloody stools and can lead to serious complications, specifically haemolytic uremic syndrome (HUS). HUS is a result of the toxin entering the blood and destroying red blood cells (haemolysis). It can lead to kidney failure (uraemia or the build-up of nitrogen wastes in the blood) and can be life-threatening. Signs and symptoms include decreased frequency of urination (evidence of uraemia), fatigue, and pale skin due to haemolytic anaemia. HUS usually develops about a week after the onset of diarrhoea.

Approximately, up to 11% of people who are diagnosed with an O157 STEC infection develop HUS. Children, the elderly, and persons with weakened immune systems are at the greatest risk. However, most healthy people recover from a STEC infection within a week and do not develop HUS. Non-O157 Shiga toxin-producing E. coli can cause the same symptoms and complications. Different testing techniques are required to identify O157 and non-O157 Shiga toxin-producing E. coli.

How is the sample collected for testing?

A fresh liquid or unformed stool sample is collected in a clean dry container. The stool sample should not be contaminated with urine or water. Once it has been collected, the stool should be taken to the laboratory immediately or refrigerated and taken to the lab as soon as possible. Some laboratories provide transport media to support the survival of the organism from the time of collection until delivery to the laboratory. STEC become difficult to detect in the stool after one week of illness, so the timing of sample collection relative to the onset of illness is important.

Is any test preparation needed to ensure the quality of the sample?

No test preparation is needed.

Accordion Title
Common Questions
  • How is it used?

    These tests are used to detect the presence of Escherichia coli (E. coli) that produce Shiga toxin and to help diagnose an infection of the digestive tract due to these bacteria. E. coli bacteria are part of healthy digestive systems in humans and other mammals, but there are strains of E. coli that produce poisons, called Shiga toxins. In addition to severe diarrhoea, Shiga toxin-producing E. coli (STEC) can cause haemolytic uremic syndrome (HUS), a serious illness that may lead to kidney failure and even death if not treated properly. Tests for STEC are used to make an accurate diagnosis and help guide treatment.

    These tests may also be used to help recognize and track suspected outbreaks of STEC. Infections are often linked to the consumption of contaminated food or water, contact with farm animals or their environment, or person-to-person contact. E. coli O157:H7 is the strain that is most common in foodborne E. coli outbreaks in the UK. However, there are non-O157 strains of STEC that can also cause severe diarrhoea and HUS, such as E. coli O104:H4.

    It is important that STEC infections be diagnosed quickly to prevent the bacteria from spreading throughout the community and so that interventions can be made, if necessary, to prevent HUS. UK Health Security Agency recommends that all diarrhoeal stools are screened for the presence of E. coli O157 (STEC).
    Tests for STEC include:

    • Stool culture: Cultures of stool samples use special nutrient media that selectively allow pathogens to grow while inhibiting growth of bacteria that are normally present in the digestive tract (normal flora). Once a pathogen grows in culture, other tests are performed to identify it. A stool sample may also be cultured for other pathogens, such as Salmonella, Shigella, and Campylobacter. O157:H7 STEC can be distinguished from other pathogens, including other types of E. coli, in culture because the bacteria have a distinct appearance when they are grown on a specific culture medium. Non-O157 STEC cannot be detected with the standard stool culture; they require special testing. Detection of non-O157 STEC is not usually performed at clinical laboratories but may be performed at public health laboratories
    • Toxin test: This test is used to detect the Shiga toxin directly using enzyme immunoassay (EIA). Stool cultures detect O157 STEC but do not detect non-O157 STEC. This test is mostly replaced by PCR test.
    • PCR test – since 2013 many local laboratories are testing the samples by a PCR test. PCR detects the genetic material of the bacteria, for example the genes that produces toxins (stx1 & stx2). Some PCR methods, especially those in the reference laboratory may look for additional genetic material. This test is extremely sensitive. The PCR test is usually followed up by culture or further tests in the reference laboratory
    • Typing methods – Public health or the reference laboratory may perform many other tests which may help to manage an outbreak. These tests try to find out if STEC obtained from two different people matches with each other. If STECs from a suspected outbreak matches with each other an outbreak could be confirmed. These tests are called typing methods. Some examples are - Phage typing, Pulsed Field Gel Electrophoresis (PFGE), Multilocus Sequence Typing (MLST), and Whole Genome Sequencing (WGS).
  • When is it requested?

    These tests may be requested when a person has diarrhoea and the doctor suspects an infection of Shiga toxin-producing E. coli Some signs and symptoms include:

    • Diarrhoea that is severe or acute and persists for more than a few days
    • Bloody stool
    • Fever
    • Severe abdominal pain, cramping and/or bloating

    Doctors or public health teams may request the test when a person presents with signs or symptoms of Haemolytic Uraemic syndrome (HUS).

    These tests are often requested when a community-wide E. coli outbreak is suspected; for example, when several people who have eaten the same food from the same source have similar signs and symptoms. Public health (UKHSA) may request a test for someone who had close contact with a patient with a STEC infection or HUS.

  • What does the test result mean?

    Stool culture
    A positive culture for STEC means that Shiga toxin-producing E. coli (likely Escherichia coli O157:H7) was detected and it is probably responsible for your signs and symptoms.
    A negative culture suggest that the E coli O157:H7 is most likely not present. Unfortunately, the culture in a local laboratory is often unable to detect other types of STEC, which are called non-O157 STECs. If your signs or symptoms suggest that a STEC infection is possible, your local laboratory would refer the sample to a reference laboratory for further testing.

    Polymerase Chain reaction (PCR)
    Many local laboratories now test the sample by PCR first. The PCR usually detects the gene responsible for toxin production or some other genetic material of the bacteria. It is an extremely sensitive test.

    PCR is able to detect both E coli O157 and non-O157 STECs. The local laboratory usually performs culture on those samples which are positive to check if this is a E coli O157, which is the commonest type in the UK. They will also send this sample to a reference laboratory for further testing.

    A negative PCR may suggest that STEC infection is most likely absent, but if your signs and symptoms are strongly suggestive of a STEC infection or HUS, your sample could be send to the reference laboratory to confirm.

  • Is there anything else I should know?

    E coli O157 and other STECs are extremely infectious as only a small number of bacteria (<100) is required to cause infection. It is known to cause outbreaks.

    Your local laboratory and clinicians must inform public health about any suspected or confirmed STEC infection or HUS. Public health may contact you to collect more information necessary to contain the infection.

    Most people must isolate until 48 hours after they have stopped vomiting and having diarrhoea. However, some people, like children or those who prepare or serve unwrapped food, may need to isolate longer, if advised to do so by the public health.

  • How are Shiga toxin-producing E. coli infections of the digestive tract treated?

    The recommended treatment for O157 Shiga toxin-producing E. coli (STEC) infection that has not progressed to haemolytic uremic syndrome (HUS) is supportive care (rest and rehydration). Those who are at an increased risk of developing HUS, such as children or the elderly, will be monitored closely. If they develop HUS, hospitalisation will be required.

    Infections of non-O157 Shiga toxin-producing E. coli are also treated with rest and rehydration, and they are often resolved without any additional intervention.

  • Why aren't antibiotics a recommended treatment for an infection with Shiga toxin-producing E. coli?

    If you are infected with Shiga toxin-producing E. coli, antibiotics may increase your risk for developing haemolytic uremic syndrome (HUS).

  • Why shouldn't I take anti-diarrhoeal medication if I have a Shiga toxin-producing E.coli infection of the gastrointestinal system?

    Anti-diarrhoeal medicines may worsen or prolong your illness because they delay the removal of E. coli from your gastrointestinal tract by inhibiting the normal movement of food and fluids through the GI tract.