Also Known As
E. coli
E. coli O157
E. coli non-O157
Formal Name
E. coli O157 culture; Shiga-like toxin by EIA; Stx gene detection by PCR
This article was last reviewed on
This article waslast modified on 25 October 2018.
At a Glance
Why Get Tested?

To determine if your gastrointestinal symptoms are due to an infection caused by Escherichia coli bacteria that produce Shiga toxin

When To Get Tested?

When you have acute diarrhoea that is persistent, severe and/or bloody

Sample Required?

A fresh liquid or unformed stool sample that does not contain urine or water, collected in a clean dry container; a rectal swab may be collected from infants. Stool and rectal swab may be placed in transport media for delivery to laboratory.

Test Preparation Needed?


On average it takes 7 working days for the blood test results to come back from the hospital, depending on the exact tests requested. Some specialist test results may take longer, if samples have to be sent to a reference (specialist) laboratory. The X-ray & scan results may take longer. If you are registered to use the online services of your local practice, you may be able to access your results online. Your GP practice will be able to provide specific details.

If the doctor wants to see you about the result(s), you will be offered an appointment. If you are concerned about your test results, you will need to arrange an appointment with your doctor so that all relevant information including age, ethnicity, health history, signs and symptoms, laboratory and other procedures (radiology, endoscopy, etc.), can be considered.

Lab Tests Online-UK is an educational website designed to provide patients and carers with information on laboratory tests used in medical care. We are not a laboratory and are unable to comment on an individual's health and treatment.

Reference ranges are dependent on many factors, including patient age, sex, sample population, and test method, and numeric test results can have different meanings in different laboratories.

For these reasons, you will not find reference ranges for the majority of tests described on this web site. The lab report containing your test results should include the relevant reference range for your test(s). Please consult your doctor or the laboratory that performed the test(s) to obtain the reference range if you do not have the lab report.

For more information on reference ranges, please read Reference Ranges and What They Mean.

What is being tested?

These tests detect the presence of disease-causing (pathogenic) Escherichia coli that produce Shiga toxins. E. coli bacteria commonly occur in nature and they are a necessary component of the digestive process in humans and most other mammals. Most strains of E. coli are harmless, but pathogenic E. coli can be responsible for inflammation of the stomach and intestines (gastroenteritis). There are multiple subtypes of E. coli that cause diarrhoeal illness, and they are classified by the mechanisms by which they cause disease. For example, some invade the lining of the intestines, causing inflammation, while others produce toxins.

E. coli that produce poisons called Shiga toxins are generally the only type of E. coli that are tested for in clinical settings from stool specimens. The Shiga toxins associated with these infections are so called because they are related to the toxins produced by another type of disease-causing bacteria, Shigella.

Outbreaks have been linked to the consumption of contaminated food, including undercooked minced beef, unpasteurised juice, raw milk, and raw produce such as leafy greens and alfalfa sprouts. Shinga toxin producing E. Coli (STEC) may also be transmitted through contaminated water, contact with farm animals or their environment, and from person to person.

In addition to symptoms of nausea, severe abdominal cramps, watery diarrhoea, fatigue, or possible vomiting and low grade fever, STEC infections are often associated with bloody stools and can lead to serious complications, specifically hemolytic uremic syndrome (HUS). HUS is a result of the toxin entering the blood and destroying red blood cells (haemolysis). It can lead to kidney failure (uraemia or the build up of nitrogen wastes in the blood) and can be life-threatening. Signs and symptoms include decreased frequency of urination (evidence of uraemia), fatigue, and pale skin due to haemolytic anaemia. HUS usually develops about a week after the onset of diarrhoea.

Approximately, up to 7% of people who are diagnosed with an O157 STEC infection develop HUS. Children, the elderly, and persons with weakened immune systems are at greatest risk. However, most healthy people recover from a STEC infection within a week and do not develop HUS. Non-O157 Shiga toxin producing E. coli can cause the same symptoms and complications. Different testing techniques are required to identify O157 and non-O157 Shiga toxin-producing E. coli.

How is the sample collected for testing?

A fresh liquid or unformed stool sample is collected in a clean dry container. The stool sample should not be contaminated with urine or water. Once it has been collected, the stool should be taken to the laboratory immediately or refrigerated and taken to the lab as soon as possible. Some laboratories provide transport media to support the survival of the organism from the time of collection until delivery to the laboratory. STEC become difficult to detect in the stool after one week of illness, so the timing of sample collection relative to the onset of illness is important.

Is any test preparation needed to ensure the quality of the sample?

No test preparation is needed.

Accordion Title
Common Questions
  • How is it used?

    These tests are used to detect the presence of Escherichia coli (E. coli) that produce Shiga toxin and to help diagnose an infection of the digestive tract due to these bacteria. E. coli bacteria are part of healthy digestive systems in humans and other mammals, but there are strains of E. coli that produce poisons, called Shiga toxins. In addition to severe diarrhoea, Shiga toxin-producing E. coli (STEC) can cause haemolytic uremic syndrome (HUS), a serious illness that may lead to kidney failure and even death if not treated properly. Tests for STEC are used to make an accurate diagnosis and help guide treatment.

    These tests may also be used to help recognize and track suspected outbreaks of STEC. Infections are often linked to the consumption of contaminated food or water, contact with farm animals or their environment, or person-to-person contact. E. coli O157:H7 is the strain that is most common in foodborne E. coli outbreaks in the U.K. However, there are non-O157 strains of STEC that can also cause severe diarrhoea and HUS, such as E. coli O104:H4.

    It is important that STEC infections be diagnosed quickly to prevent the bacteria from spreading throughout the community and so that interventions can be made, if necessary, to prevent HUS. Tests for STEC include:

    • Stool culture: Cultures of stool samples use special nutrient media that selectively allow pathogens to grow while inhibiting growth of bacteria that are normally present in the digestive tract (normal flora). Once a pathogen grows in culture, other tests are performed to identify it. A stool sample may also be cultured for other pathogens, such as Salmonella, Shigella, and Campylobacter. O157:H7 STEC can be distinguished from other pathogens, including other types of E. coli, in culture because the bacteria have a distinct appearance when they are grown on a specific culture medium. Non-O157 STEC cannot be detected with the standard stool culture; they require special testing. Detection of non-O157 STEC is not usually performed at clinical laboratories but may be performed at public health laboratories. The Health Protection Agency (HPA) recommends that a culture for STEC be performed on all stools submitted for culture from people with acute, community-acquired diarrhoea. Community-acquired diarrhoea is in contrast to diarrhoea acquired in a health care setting, such as a hospital.
    • Toxin test: This test is used to detect the Shiga toxin directly using enzyme immunoassay (EIA). Stool cultures detect O157 STEC but do not detect non-O157 STEC. Therefore, the HPA recommends that EIA to detect Shiga toxins be used if stool cultures are negative. Enzyme immunoassay only identifies the presence of Shiga toxins and does not determine which strain of E.coli is producing the toxin.
    • Pulsed-field Gel Electrophoresis (PFGE): This method is used by public health laboratories to identify subtypes of E. coli that are suspected in an outbreak. It creates a DNA "fingerprint" of the bacteria detected that is entered into a national database to be compared to other fingerprints. If matches are made, it may indicate occurrences of disease caused by the same strain of bacteria. In this way, public health labs can quickly evaluate the cause of an outbreak even though the illnesses might occur in different geographic areas.
    • Genetic tests: PCR for Shiga toxin 1gene (stx1) and Shiga toxin 2 gene (stx2) are rapid molecular tests that can be used to confirm the presence of STEC. 
  • When is it requested?

    These tests may be requested when a person has diarrhoea and the doctor suspects an infection of Shiga toxin-producing E. coli.

    Some signs and symptoms include:

    • Diarrhoea that is severe or acute and persists for more than a few days
    • Bloody stool
    • Fever
    • Severe abdominal pain, cramping and/or bloating

    These tests are often requested when a community-wide E. coli outbreak is suspected; for example, when several people who have eaten the same food from the same source have similar signs and symptoms.

  • What does the test result mean?

    Stool culture
    A negative stool culture for Shiga toxin-producing Escherichia coli (STEC) means that Escherichia coli O157:H7 was not present or was not present in sufficient numbers to be detected. The culture results may indicate that a pathogen other than E. coli is causing the symptoms. These could include the bacterial pathogens Salmonella, Shigella, and Campylobacter, viral pathogens, or parasites. A positive culture for STEC means that Shiga toxin-producing E. coli (likely Escherichia coli O157:H7) was detected.

    Toxin test by EIA
    A negative result for enzyme immunoassay (EIA) for Shiga toxin suggests that the toxin is not present.

    A positive result for enzyme immunoassay (EIA) for Shiga toxins suggests that the toxins are present in the stool and that further testing should be done to grow and identify the E. coli producing the toxins.


  • Is there anything else I should know?

    Non-pathogenic E. coli are a normal part of a healthy human digestive system. However, E. coli infections in parts of the body other than the gastrointestinal system can cause illness. E. coli is responsible for the majority of urinary tract infections and can also cause neonatal meningitis, among other infections. These E.coli infections are detected by culturing a sample from the infected area.

  • How are Shiga toxin-producing E. coli infections of the digestive tract treated?

    The recommended treatment for O157 Shiga toxin-producing E. coli (STEC) infection that has not progressed to haemolytic uremic syndrome (HUS) is supportive care (rest and rehydration). Those who are at an increased risk of developing HUS, such as children or the elderly, will be monitored closely. If they develop HUS, hospitalisation will be required.

    Infections of non-O157 Shiga toxin-producing E. coli are also treated with rest and rehydration, and they are often resolved without any additional intervention.

  • Why aren't antibiotics a recommended treatment for an infection with Shiga toxin-producing E. coli?

    If you are infected with Shiga toxin-producing E. coli, antibiotics may increase your risk for developing haemolytic uremic syndrome (HUS).

  • Why shouldn't I take anti-diarrhoeal medication if I have a Shiga toxin-producing E.coli infection of the gastrointestinal system?

    Anti-diarrhoeal medicines may worsen or prolong your illness because they delay the removal of E. coli from your gastrointestinal tract by inhibiting the normal movement of food and fluids through the GI tract.