von Willebrand Factor

Also Known As

VWF:Ag

VWF:RCo

von Willebrand Panel

Ristocetin Cofactor

Formal Name

von Willebrand Factor Antigen and von Willebrand Factor Activity

This article was last reviewed on

6 October 2023.

This article waslast modified on 6 October 2023.

At a Glance

Why Get Tested?

To help determine the cause of unexplained excessive or repeated episodes of bleeding, to diagnose von Willebrand disease (VWD), and to distinguish between different types of VWD

When To Get Tested?

When you have a personal or family history of heavy, prolonged, and/or spontaneous bleeding; when your healthcare professional suspects that you may have a bleeding disorder

Sample Required?

The test is performed on a sample of blood obtained from a vein in the arm

Test Preparation Needed?

No test preparation is needed

On average it takes 7 working days for the blood test results to come back from the hospital, depending on the exact tests requested. Some specialist test results may take longer, if samples have to be sent to a reference (specialist) laboratory. The X-ray & scan results may take longer. If you are registered to use the online services of your local practice, you may be able to access your results online. Your GP practice will be able to provide specific details.

If the doctor wants to see you about the result(s), you will be offered an appointment. If you are concerned about your test results, you will need to arrange an appointment with your doctor so that all relevant information including age, ethnicity, health history, signs and symptoms, laboratory and other procedures (radiology, endoscopy, etc.), can be considered.

Lab Tests Online-UK is an educational website designed to provide patients and carers with information on laboratory tests used in medical care. We are not a laboratory and are unable to comment on an individual's health and treatment.

Reference ranges are dependent on many factors, including patient age, sex, sample population, and test method, and numeric test results can have different meanings in different laboratories.

For these reasons, you will not find reference ranges for the majority of tests described on this web site. The lab report containing your test results should include the relevant reference range for your test(s). Please consult your doctor or the laboratory that performed the test(s) to obtain the reference range if you do not have the lab report.

For more information on reference ranges, please read Reference Ranges and What They Mean.

What is being tested?

Von Willebrand factor (vWF or VWF) is a protein that is one of several components of the coagulation system that work together, and in sequence, to stop bleeding within the body. VWF testing measures the amount of the protein present in blood (VWF antigen) and determines how well the protein functions (VWF activity).

Normally, when a blood vessel is damaged and bleeding begins, VWF forms an adhesive bridge between activated cell fragments called platelets and the injury site. This is followed by the clumping (aggregation) of platelets at the site and a series of actions referred to as activation of the coagulation cascade, resulting in the formation of a stable blood clot.

VWF further affects clotting by influencing the availability of coagulation factor VIII, an important protein in blood clot formation. VWF carries factor VIII in the blood, increases its half-life, and releases it as necessary. If the amount of functional VWF is insufficient, then platelet adherence and aggregation are affected, levels of factor VIII could be decreased, blood clot formation takes longer, and therefore bleeding is prolonged. This deficiency causes a condition referred to as von Willebrand disease (VWD).

VWD is the most common inherited bleeding disorder. It is a group of conditions associated with prolonged bleeding due to deficient and/or defective VWF. VWD is separated into different types and sub-types, including:

Type 1 – with this type of VWD, there is a decrease in the amount of VWF produced, but the VWF functions normally. Levels of factor VIII are also typically low but may be normal. This is the most common type of VWD, accounting for about 75% of cases. It tends to cause bruising and mild to moderate bleeding, such as persistent nosebleeds, heavy menstrual periods, and prolonged bleeding following childbirth, trauma, dental procedures, and surgeries. Symptoms and the severity of bleeding will vary from person to person and from episode to episode.
Type 2 – this type is associated with a normal amount of VWF, but the VWF does not function normally. i.e. VWF antigen may be normal but VWF activity will be reduced. Bleeding may be more severe with this type. Type 2 is further divided into Types 2A, 2B, 2M, and 2N based on the specific function affected. Further specialised testing is often required to determine the subtype of Type 2 VWD.
Type 3 – this rare type is associated with very little VWF production, very low factor VIII levels, and moderate to severe symptoms. It is often detected in infancy because of early bleeding episodes.

Rarely, VWD may be due to an acquired VWF deficiency, where there is no family or personal bleeding history up to the point when signs and symptoms develop. (For more on this, see Common Questions #7.)

Von Willebrand factor testing includes VWF antigen, which measures the amount of VWF, and VWF activity (sometimes known as Ristocetin Cofactor), which evaluates the function of VWF. Some laboratories may offer a panel that includes both of these tests along with a factor VIII activity test.

Common Questions

How is it used?
Von Willebrand factor (VWF) testing is used to investigate excessive or recurrent bleeding episodes or a personal or family history of excessive bleeding. Testing is used to help diagnose von Willebrand disease (VWD) and distinguish between the various types of VWD.

VWF is a protein, one of several components of the coagulation system that work together to stop bleeding and form a stable blood clot. If there is insufficient VWF or if it does not function properly, bleeding may be excessive or prolonged. This is called von Willebrand disease and it is the most common inherited bleeding disorder. VWD is separated into different types: Type 1, Type 2, and Type 3. Type 1 is the most common and tends to be milder than the other types. Rarely, VWD may be due to an acquired VWF deficiency (see Common Questions #7).

Two types of tests may be used:

VWF antigen – this test measures the amount of the VWF protein present in the blood

VWF activity (sometimes called Ristocetin Cofactor) – this test determines whether the protein is functioning properly.

These tests may be requested by themselves or along with a coagulation factor VIII activity test and following other bleeding disorder tests, such as a full blood count (FBC), platelet count, platelet function tests (e.g., platelet aggregation, etc.), PT (prothrombin time), and/or PTT (partial thromboplastin time).

Other tests may be requested following VWF testing for more information and to distinguish between subtypes. These may include:

Ratio of VWF:RCo to VWF:Ag

Factor VIII binding assay

Platelet VWF studies

Collagen binding activity assay

Ristocetin-induced platelet aggregation (RIPA) or platelet binding

VWF propeptide (VWFpp) to VWF antigen ratio

Molecular genetic testing for VWD (e.g., to confirm Type 2N and Type 3)

Multimeric analysis – VWF is a protein complex that exists as "multimers" of different size. This test looks at the distribution of different sizes to help distinguish between Type 2 subtypes.

Blood group
When is it requested?
VWF testing is requested after initial screening tests for a bleeding disorder (such as platelet function tests, PT, PTT) have been performed to investigate someone's personal or family history of excessive or recurrent bleeding episodes. The signs and symptoms that may prompt testing vary depending on the type of VWD an individual has and may include:

Frequent or repeated nose bleeds

Excessive bleeding from the gums after dental procedures

Excessive bruising after minor knocks or injuries

Heavy and/or prolonged menstrual bleeding in women

Blood in urine or stool

Prolonged bleeding after surgery

VWF tests may be repeated when they are initially normal but suspicions of VWD remain high.

When VWF testing suggests VWD, additional testing may be performed to determine which subtype the person has.
What does the test result mean?

Interpretation of VWF test results can be challenging and may require consultation with a doctor who specialises in bleeding disorders, such as a haematologist or coagulation specialist, especially when determining subtypes. People who do not have VWD may have moderately decreased test results particularly those with type O blood group.

In a person with normal or near normal bleeding disorder screening test results, a significantly decreased VWF antigen test suggests that the person tested has a quantitative VWF deficiency and may have Type 1 VWD or, more rarely, may have acquired VWD.

If the VWF antigen test is normal or nearly normal and the VWF activity (Ristocetin Cofactor) is decreased, then the person may have Type 2 VWD. Further testing (e.g., VWF multimeric analysis) will be required to determine which subtype is present. In Type 2N, both VWF antigen and activity may be normal but factor VIII will be low. These test results are similar to those obtained in haemophilia A (a factor VIII deficiency bleeding disorder that affects males) and therefore further testing will be required to distinguish between the 2 disorders. This may involve factor VIII binding assays or genetic testing.

If no or very little VWF and factor VIII are present, then the person may have Type 3 VWD. This will typically be seen in a child who experiences bleeding episodes early in life.

Increased concentrations of VWF antigen and VWF activity are not considered diagnostic. VWF is one of many acute phase reactants. This means that concentrations will be temporarily increased with infections, inflammation, trauma, and with physical and emotional stressors. They are also increased with pregnancy and with the use of oestrogen medications such as oral contraceptives.
Is there anything else I should know?

A person's ABO blood type affects VWF concentrations. People with type O blood have VWF levels that are up to 25% lower than those with other blood types.

Measuring concentrations of other acute phase reactants such as CRP and fibrinogen may be helpful if VWF concentrations are borderline normal. For example, a borderline normal VWF with significantly high CRP and/or fibrinogen may suggest VWD.

VWF is produced by megakaryocytes and by the endothelial cells that line blood vessels. It is released by platelets and endothelial cells as needed.
Should everyone be tested for von Willebrand factor (VWF)?

No, VWF tests are not intended to be used for general screening or for initial bleeding disorder investigation. Most people will never need to have VWF testing performed.
Can VWF testing be performed in my doctor's surgery?

VWF testing must be performed in a laboratory and is often sent to a reference laboratory. It requires specialised equipment and interpretation.
If I have heavy menstrual periods, do I have von Willebrand disease (VWD)?

It is possible, but many women who experience heavy bleeding during menstruation do not have VWD.
Can I have VWD and not know it?

Yes. Some people experience no or few symptoms, even though they have quantitative or qualitative VWF deficiencies.
Should I tell my other doctors that I have been diagnosed with VWD?

Yes. This is important information for your health care providers, including your dentist, to have. They will need to plan ahead for any surgeries, childbirths, or dental procedures you may have.
Is there treatment for VWD?

Yes, there is treatment that can be given intermittently, primarily before procedures that may trigger bleeding. There is a medication called desmopressin (DDAVP) that promotes the release of VWF in some people with VWD; there are also VWF/FVIII concentrate replacement therapies; and there are other non-VWF related measures that can be taken to control bleeding.
Are there other causes of VWF deficiency besides inherited causes?
Yes, a deficiency in VWF may be due to another disease or condition, where there is no family or personal bleeding history up to the point when signs and symptoms develop, but this rarely occurs. It is sometimes seen in people with:

Conditions that cause the breakdown of VWF, such as increased pressure in the arteries leading from the heart to the lungs (pulmonary hypertension) and structural defects of the heart (e.g., aortic valve stenosis)

Lymphoma, myeloma, or autoimmune disorders (such as systemic lupus erythematosus) that cause the production of VWF antibodies

Myeloproliferative neoplasms associated with increased platelet production that cause increased binding of VWF to platelets

Hypothyroidism, which can decrease VWF production

Wilms tumor and other disorders that bind to VWF and remove it from the blood

Certain medications such as valproic acid, ciprofloxacin, hetastarch

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