To find out if a person has drunk alcohol and to measure the amount of alcohol present in the body
When a patient has symptoms that suggest drunkenness or alcohol toxicity, or when a person is suspected of breaking drinking-related laws or as part of a drug testing panel for pre-employment or other purposes.
Ethanol (Ethyl alcohol) can be measured in a blood or urine sample, in breath or (rarely) in saliva. Blood, urine, and saliva samples must be sent to a laboratory for analysis. A breath sample is analysed immediately on site using a breath analyser ("breathalyser").
This test measures the amount of ethanol in the blood, urine, breath, or saliva. Ethanol (also called ethyl alcohol or alcohol) is the intoxicating ingredient in alcoholic beverages such as beer, wines and spirits. Small amounts of alcohol can cause excitement, relaxation, and decreased inhibition, but can also cause poor judgment and impaired eye-hand coordination; large amounts in a relatively short period of time can cause rapid alcohol poisoning with confusion, slow breathing, coma, and even death. Consumption of large quantities of alcohol over a long period of time can lead to alcoholism and to several medical problems such as permanent liver damage, cardiovascular problems, depression and anxiety.
When alcohol is drunk, it is absorbed by the stomach and intestine, and carried throughout the body in the bloodstream. Small amounts of alcohol are removed by the kidney in urine or breathed out from the lungs, but most is broken down by the liver. Alcohol is poisonous to the liver. With the help of enzymes, the liver oxidizes the alcohol first to acetaldehyde, then to acetate, and then finally to carbon dioxide and water. The liver can process about one unit of alcohol an hour – with one unit of alcohol being defined in the UK as 10 mL pure alcohol, roughly equivalent to 250 mL (half a can) of beer, 80 mL (half a 175 mL glass) of wine, or a measure (25 mL) of whisky. A person who drinks more than 1 drink an hour is likely to build up alcohol in their blood stream.
How is the sample collected for testing?
A blood sample is obtained by inserting a needle into a vein in the arm; a breath sample is collected by blowing into a tube attached to a measuring device. Urine samples are collected in plastic containers; sometimes a single urine sample is collected and sometimes two separate samples may be collected with the first discarded and the second collected after a measured time. Saliva samples may be collected from the mouth using a swab.
Is any test preparation needed to ensure the quality of the sample?
No test preparation is needed. Breath alcohol concentrations may be invalid if alcohol has been consumed orally within 15 minutes of the test due to the presence of alcohol in the mouth.
How is it used?
The ethanol/alcohol test is used for both medical and legal purposes. Samples and results for each use are usually collected and tested separately.
Medical: The doctor looks for alcohol to help treat the patient's symptoms. For medical purposes, blood, and sometimes urine, alcohol tests are used to detect the presence of ethanol and to measure its concentration. One or more of these tests may be requested when a patient is seen in the Accident and Emergency department with symptoms suggesting alcohol poisoning. The symptoms may include confusion, staggering, vomiting, and unconsciousness. Other tests, such as a Full Blood Count (FBC), glucose and electrolytes are often ordered at the same time as there are a variety of other conditions that can cause similar symptoms. Additional drug testing and testing for the presence of other, more toxic, alcohols (such as methanol and isopropyl alcohol) may also be performed if the use of other substances is suspected.
Legal: Legal testing must be done by specially trained analysts and must have a strict chain-of-custody (a paper trail that records sample movement and handling). Testing may be requested to determine whether an erratic driver has a blood alcohol concentration that is over a legal limit, to determine whether an under-age child has been drinking, or to find out whether drinking alcohol has contributed to an accident. Post-mortem ethanol testing may be done to discover whether alcohol contributed to a person’s death. Legal ethanol testing may also be done randomly or as part of an employer's drug testing programme to see whether an employee has alcohol in their system.
Samples tested for legal purposes may include blood, breath, urine, and/or saliva. Breath testing is the most common alcohol test performed on drivers. It uses the tail end sample of breath from deep in the lungs and uses a conversion factor to estimate the amount of alcohol in the blood. Blood alcohol testing may be requested to confirm or refute other evidence, and/or be used as an alternative to breath testing. Urine testing may also be performed as an alternative. A random urine sample is sometimes used to monitor people for the presence of alcohol. Testing saliva for the presence of alcohol is not as widely used, but may be used as an alternative screening test.
When is it requested?
Medical ethanol testing is used when a patient has symptoms that suggest intoxication and or ethanol toxicity, such as flushed face, glassy or watery eyes, slurred speech or drowsiness. Medical testing may also be done on a regular basis to ensure that a person being treated for alcoholism is not continuing to abuse alcohol.
Legal ethanol testing may be requested whenever there is reason to suspect that a person has not complied with a drinking-related law, and whenever there has been an accident and/or unexpected death – to determine whether alcohol played a role. Employment alcohol testing may be performed randomly and when the employer suspects that an employee has alcohol in their system while on the job.
What does the test result mean?
For medical testing, the detection of ethanol in a sample indicates that a person is likely to have been drinking, and the concentration present can give an indication of the severity of alcoholintoxication Symptoms and complications at a given ethanol concentration may vary significantly from person to person, however, depending on the individual’s general health, age, tolerance to alcohol and other medications or drugs that he or she is taking. The ability to remove alcohol from the body also depends on the availability of the necessary liver enzymes and a healthy liver.
For legal testing, results obtained are compared to legal allowable limits. In the UK (July 2015), the legal limit for driving is 80 milligrams/100 mL of blood, or 107 milligrams/100 mL of urine. The legal limit in breath samples is 35 micrograms of alcohol in 100 mL of breath.
Blood ethanol concentrations in the range 80 to 400 mg/100 mL cause increasing impairment and depression of the central nervous system. Concentrations above 400 mg/100mL blood are likely to reduce in loss of consciousness and may prove fatal.
Is there anything else I should know?
Different ethanol sample results are not interchangeable. Breath and urine samples are considered to be good estimates of blood alcohol concentrations in most people but can be affected by a variety of factors. Breath sample concentrations can be affected by alcohol consumption within the last few minutes, ketones (released into the breath by some diabetics and dieters), and by other substances that contain alcohol such as mouthwash and cough syrup. Urine concentrations lag behind blood concentrations. Peak concentrations in urine are reached approximately 45-60 minutes after alcohol ingestion. Urine samples that contain both glucose (such as may be seen in diabetics) and bacteria or yeast, should not be left at room temperature for long periods of time because the glucose can ferment and produce ethanol. Fermentation can also be seen in post-mortem samples. Occasionally two other tests are used which measure serotonin metabolites, 5-HIAA (5-hydroxyindoleacetic acid) and 5-HTOL (5-hydroxytryptophol), which can help confirm whether alcohol was drunk. An increased ratio of 5-HTOL/5-HIAA can indicate alcohol consumption.
Children may develop low blood sugar (hypoglycaemia) in association with alcohol poisoning, so health practitioners may request blood glucose tests along with ethanol concentrations if they suspect ethanol toxicity in children.
Is there anything I can do to get rid of the alcohol in my system faster?
Are medical ethanol results released for legal purposes?
Can I choose which sample will be collected?
Does everyone metabolise alcohol at the same rate?